A Comparative Genomic Analysis of Georgenia sps. for Mining of LysR Transcriptional Regulator Sequences

Tejas Oza; Pooja Patel; Vrinda S. Thaker

doi:10.61797/ijbic.v3i1.285

Tejas Oza Plant Biotechnology and Genetic Engineering Laboratory, Department of Biosciences, Saurashtra University, Rajkot 360005, Gujarat, India
Pooja Patel Plant Biotechnology and Genetic Engineering Laboratory, Department of Biosciences, Saurashtra University, Rajkot 360005, Gujarat, India
Vrinda S. Thaker Plant Biotechnology and Genetic Engineering Laboratory, Department of Biosciences, Saurashtra University, Rajkot 360005, Gujarat, India

DOI: https://doi.org/10.61797/ijbic.v3i1.285

Keywords: Comparative genomics, Georgenia sp.SUBG003, Genomic islands, LysR transcriptional factors, Phylogeny, Pathogenicity

Abstract

Georgenia is a genus belonging to the actinomycetes group. The genera comprise only 33 poorly characterized species with reference genomes of 10 distinct species. However, none of the species is well characterized for their genome characteristics. Our laboratory isolate from tomato plant leaf was identified and sequenced for identification and found to be Georgenia sp. Later genomic analysis revealed many functional genes having characteristic functions to be analysed. The source of isolation raised the possibility of having functional genes to enhance senescence or having plant pathogenic activity by Georgenia sp. SUBG003. To explore in silico presence of these genes or gene pools genomic islands were identified and analysed for our isolate and other 10 reference genomes of the Georgenia genus. Genomic islands were further explored for transcription regulators and finally, LysR transcriptional regulator sequences were extracted and a phylogeny among sequences was built from multiple sequence alignment.